Taxol is a complicated diterpene alkaloid with anti-tumor activity, which was first isolkated from Taxos brevifolia by Wani et

The effects of some factors on the preparation and regeneration of protoplasts from the taxol-producing fungus Nodulisporium sylviforme were discussed in this paper, including recombination of various enzymes, digesting time and temperature, and pH value. The results showed that the highest preparation frequency of protoplasts is a concentration of 2.0×10 protoplasts per microlitre. The mutagensis of the protoplasts from Nodulisporium sylviforme mycelia induced with UV or UV-0.6% LiCl were carried out. The condition of the best mutagensis was achieved when the protoplasts of Nodulisporium sylviforme were irradiated by a 30 w UV lamp in a 30 cm distance for the 40 s. By primary and secondary screen, the mutant that the yield of taxol raised 25.01% (from 314.07 μg/L to 392.63 μ g/L) was obtained. In addition, we made a study on analysis of peroxidase isozyme of taxol-producing fungus parent strain NCEU-1 and its mutant. [The Journal of American Science. 2005;3(1):55-62].